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C3 Single Cell Sequencing Workshop Group

Hosts

Dr. Peter Adams, Sanford Burnham Prebys Medical Discovery Institute

Dr. Geoffrey Wahl, Salk Institute for Biological Studies

Dr. Brian James, Sanford Burnham Prebys Medical Discovery Institute 

 

Single Cell Sequencing Workshop Group/Social

Date: First Monday Monthly

Time:  5:00pm - 6:00pm

Location: 

Sanford Burnham Prebys Medical Discovery Institute

Fishman Auditorium, Building #4

10901 North Torrey Pines Road

La Jolla, CA 92037

  • Free Parking, See Map

 

2017 Workshop Group/Social Dates

Monday, March 6, 2017
 
Monday, April 3, 2017
 
Monday, May 1, 2017
 
Monday, June 5, 2017:
Presenter: Brandon Sos from Kun Zhang’s lab at UCSD; Title: The Single Cell Transposome Hypersensitive Sites Sequencing (scTHS-seq) assay for Chromatin Accessibility and Assessment of Epigenetic States in the Human Adult Brain; 
Summary: Chromatin accessibility captures in vivo protein-chromosome binding status, and is considered an informative proxy for protein-DNA interactions. Current single cell chromatin accessibility assays result in sparse chromatin maps at high throughput or dense chromatin maps at low throughput. We present a high throughput transposome hypersensitive sites sequencing assay for highly sensitive characterization of chromatin accessibility in single cells, generating thousands of single cell datasets from the human adult visual and frontal cortex. Integrative analysis of transcriptomic snDrop-seq data and scTHS-seq data has allowed us to identify transcription factors and regulatory elements shaping the state of different brain cell types, and to map genetic risk factors of common human brain diseases to specific pathogenic cell types and subtypes.
Monday, August 7, 2017:
Presenter: Chongyuan Luo
Monday, September 4, 2017
 
Monday, October 2, 2017:
Presenter: Zhuzhu Zhang
Monday, November 6, 2017:
Presenter:  Sara Linker; Title: Single-nucleus RNA-seq (snRNA-seq) reveals a unique transcriptional response in the dentate gyrus in response to hippocampal activation; 
Summary: Dentate granule cells (DGCs) are sparsely active within the mouse hippocampus. Through snRNA-seq we have examined the transcriptional response of hippocampal neurons to activity and identified that, despite their rare probability of activation, DGCs have an exaggerated activity-induced transcriptional response compared to other hippocampal populations. These results highlight the importance of the baseline cell state in determining the transcriptional response to neural activity. 
Monday, December 4, 2017
 




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