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Calendar: C3-SCS: Single Cell Sequencing Group Workshop Group/Social

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Title

C3-SCS: Single Cell Sequencing Group Workshop Group/Social

Location

SBP: Fishman Auditorium

Start Time

6/3/2019 4:00 PM

End Time

6/3/2019 5:00 PM

Type of Event

C3 Event

Description

Monday, June 3, 2019 4:00pm-5:00pm Sanford Burnahm Prebys Medical Discovery Institute Fishman Auditorium (parking is free) * All are welcome and light hors d'oeuvres/drinks will be served Hosts: Drs. Peter Adams, Brian James and Geoffrey Wahl Speaker/Topic for Discussion are as follows: Presenter: Herbert Tseng, Ph.D., Senior Staff Researcher, Kaech Lab at Salk Institute for Biological Studies Title: The Single Cell Barcode Chip (SCBC), a high-throughput multiplex assay for single-cell secretome analysis Summary: Signaling through the secretion of cytokines, chemokines, and growth factors is a critical regulatory function by which cells can influence the behavior of surrounding cells and the environment around it. The functional secretome of a cell can reveal important information on cellular behavior in diseased states like tumors, and their phenotypic distribution within a tissue, such as with various immune cell subsets. That cell secretion is heterogeneous even within classically defined subpopulations, particularly polyfunctional cells that secrete multiple effector proteins, necessitates its analysis on a single cell level. Traditionally, ELISPOT assays would be used to detect the secretome, but these assays are limited in the number of proteins that can be studied, as well as throughput. Flow cytometry with intracellular cytokine staining requires fixation, and thus is not necessarily a true analysis of cell secretion. Given these limitations, the Kaech Lab has helped to develop a high-throughput multiplex single-cell secretome assay known as the Single Cell Barcode Chip (SCBC). In the SCBC, we capture single cells in PDMS nanowells that secrete onto a barcoded ELISA microarray. Using this platform, we can detect up to 15 secreted proteins of thousands of cells at a single cell level to provide a functional profile of the input cells. Dr. Tseng will discuss the development of the SCBC assay to its current form, and his progress in implementing this assay at the Salk Institute in the Kaech Lab. Additionally, please mark your calendars for the First Monday Monthly from 4p-5p @ SBP-Fishman Auditorium for future meetings and for more information: http://sdcancercouncil.org/C3SCS/SitePages/Home.aspx

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Created at 5/31/2019 10:20 AM by FREELANDER\c3_admin
Last modified at 5/31/2019 10:20 AM by FREELANDER\c3_admin